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Rhodamine B doped silica nanoparticle labels for protein microarray detection |
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| Authors: | WANG Ying LI ZhongHui ZHONG WenYing LI Hui XU DanKe & CHEN HongYuan Key Laboratory of Analytical Chemistry for Life Science Ministry of Education School of Chemistry Chemical Engineering Nanjing University Nanjing China |
| Affiliation: | WANG Ying1,LI ZhongHui1,ZHONG WenYing2,LI Hui2,XU DanKe1 & CHEN HongYuan1 1Key Laboratory of Analytical Chemistry for Life Science,Ministry of Education,School of Chemistry , Chemical Engineering,Nanjing University,Nanjing 210093,China,2Department of Analytical Chemistry,China Pharmaceutical University,Nanjing 210009 |
| Abstract: | A core-shell Rhodamine B-doped SiO2 nanoparticle was synthesized and its fluorescent intensity was found to be 1000 times higher than that of individual Rhodamine B molecule. The doped nanoparticles were further conjugated with streptavidin and the resulting nanoparticles were used in the detection of reverse-phase protein microarrays, in which human IgG of various concentrations was first immobilized on aldehyde-modified glass slides and then biotinlyated goat anti human IgG as well as the labeled nanopart... |
| Keywords: | Rhodamine B SiO2 nanoparticles protein detection protein microarrays |
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