Carbohydrate-Derived Metal-Chelator-Triggered Lipids for Liposomal Drug Delivery |
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Authors: | Thomas Holmstrøm Mette Galsgaard Malle Shunliang Wu Knud Jørgen Jensen Nikos S Hatzakis Christian Marcus Pedersen |
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Institution: | Department of Chemistry, University of Copenhagen, Universitetsparken 5, 2100 Copenhagen, Denmark |
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Abstract: | Liposomes are versatile three-dimensional, biomaterial-based frameworks that can spatially enclose a variety of organic and inorganic biomaterials for advanced targeted-delivery applications. Implementation of external-stimuli-controlled release of their cargo will significantly augment their wide application for liposomal drug delivery. This paper presents the synthesis of a carbohydrate-derived lipid, capable of changing its conformation depending on the presence of Zn2+: an active state in the presence of Zn2+ ions and back to an inactive state in the absence of Zn2+ or when exposed to Na2EDTA, a metal chelator with high affinity for Zn2+ ions. This is the first report of a lipid triggered by the presence of a metal chelator. Total internal reflection fluorescence microscopy and a single-liposome study showed that it indeed was possible for the lipid to be incorporated into the bilayer of stable liposomes that remained leakage-free for the fluorescent cargo of the liposomes. On addition of EDTA to the liposomes, their fluorescent cargo could be released as a result of the membrane-incorporated lipids undergoing a conformational change. |
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Keywords: | carbohydrates drug delivery lipids liposomes zinc |
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