| 蛋白激酶C单克隆抗体的制备 |
| |
| 引用本文： | 李华,柳惠图.蛋白激酶C单克隆抗体的制备[J].北京师范大学学报(自然科学版),1993,29(1):137-141. |
| |
| 作者姓名： | 李华 柳惠图 |
| |
| 作者单位： | 北京师范大学生物系，北京师范大学生物系 100875 北京新外大街，100875 北京新外大街 |
| |
| 摘 要： | 应用杂交瘤技术制备了蛋白激酶C(PKC)的单克隆抗体,用蛋白A-Sepharose-CL4B协同沉淀复合物分析单抗识别的蛋白,分子质量与PKC相同。采用该抗体对正常和转化的C_3H_(10) T_(1/2)细胞进行免疫荧光观察,发现它们的PKC含量明显不同。但荧光分布都主要集中在细胞质和细胞膜部分。
|
| 关 键 词： | 蛋白激酶C 单克隆抗体 杂交瘤 |
PREPARATION OF MONOCLONE ANTIBODY AGAINST RABBIT BRAIN CYTOSOLIC PROTEIN KINASE C |
| |
| Li Hua Liu Huitu.PREPARATION OF MONOCLONE ANTIBODY AGAINST RABBIT BRAIN CYTOSOLIC PROTEIN KINASE C[J].Journal of Beijing Normal University(Natural Science),1993,29(1):137-141. |
| |
| Authors: | Li Hua Liu Huitu |
| |
| Abstract: | Hybrid clones are selected with HAT medium and tested for anti-PKC antibody by ELISA coating with purified protein kinase C(PKC). After the positive clones are subcloned by limited dilute method they were injected into pristane-primed Balb/C mice to prepare the ascites fluid. The enzyme is distributed in the plasma membrane and cytoplasm with immu- nocytochemical method using the prepared antibody and differences between C_3H_(10) T_(1/2) cell and transformed C_3H_(10) T_(1/2) cell are observed. SDS-PAGE anal- ysis of the immunoprecipitates of crude extract of C_3H_(10)T_(1/2) cell with the monoclone antibody desolved a protein with the same molecular weight of protein kinase C. |
| |
| Keywords: | protein kinase C monoclone antibody hybridroma cell fusion immunocytochemistry |
| 本文献已被 CNKI 维普 等数据库收录！ |
