聚鲁米诺-金属离子复合物膜的电化学发光特性及其分析应用研究

建立了一种基于电聚合和配位效应构建聚鲁米诺-金属离子复合物膜修饰电极测定尿素的电化学发光分析新方法, 并且提出了一种优化聚鲁米诺电化学发光分析特性的新思路. 在最佳条件下, 增敏电化学发光信号与尿素的质量浓度在2.0×10-9—1.0×10-7 g/mL 范围内呈线性关系, 检出限为1.7×10-10 g/mL.

Electrochemiluminescence Performances of Compositing Films of Polyluminol with Metal Ions and Its Analytical Application

In this paper, it was found that the compositing films made from polyluminol and some metal ions(such as Ni2+, Fe2+ and Co2+ ) can be easily generated on the surface of the graphite electrode by electropolymerization of luminol and these metal ions in the acidic medium. Compared to other resulting films, the compositing films made from polyluminol and Ni2+ presented the better electrochemiluminescence(ECL) analytical performance for urea based on its sensing effect for weak ECL signal of electro-oxidation polyluminol. On the basis of these results, a novel ECL method for the determination of urea in urine samples was developed, and a new method to improve the ECL analytical performances of polyluminol by compositing metal ions was also proposed. Under the optimum experimental conditions, the ECL response was linearly to the concentration of urea within the range of 2.0×10-9—1.0×10-7 g/mL with a detecting limit of 1.7×10-10 g/mL.