| 利用泰勒分析和动态涂层毛细管电泳系统快速测定蛋白质的扩散系数 |
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| 引用本文： | 左敏,陈义.利用泰勒分析和动态涂层毛细管电泳系统快速测定蛋白质的扩散系数[J].高等学校化学学报,2007,28(10):1875-1877. |
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| 作者姓名： | 左敏 陈义 |
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| 作者单位： | 1. 中国科学院化学研究所生命分析化学实验室,北京,100080;中国科学院研究生院,北京,100039
2. 中国科学院化学研究所生命分析化学实验室,北京,100080 |
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| 基金项目： | 国家自然科学基金
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科技部科研项目 |
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| 摘 要： | 以泰勒分散理论为基础, 首次采用动态涂层毛细管来准确和快速测定蛋白质分子的扩散系数.
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| 关 键 词： | 微量蛋白质 扩散系数 毛细管电泳 泰勒分析 |
| 文章编号： | 0251-0790（2007）10-1875-03 |
| 收稿时间： | 2007-05-21 |
| 修稿时间： | 2007-05-21 |
Fast Determination of Protein Diffusion Coefficient by Taylor Dispersion Analysis and Capillary Electrophoresis System |
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| ZUO Min,CHEN Yi.Fast Determination of Protein Diffusion Coefficient by Taylor Dispersion Analysis and Capillary Electrophoresis System[J].Chemical Research In Chinese Universities,2007,28(10):1875-1877. |
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| Authors: | ZUO Min CHEN Yi |
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| Institution: | 1. Laboratory of Analytical Chemistry for Life Science, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100080, China ; 2. Graduate School, Chinese Academy of Sciences, Beijing 100039, China |
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| Abstract: | A method for the fast and accurate measurement of diffusion coefficient of minute proteins was developed. A protein sample plug was introduced into the capillary and forced to flow through a capillary coated dynamically by poly(diallyldimethylammonium chloride). Its concentration profile was then monitored by the UV absorption detector mounted on Beckman MDQ capillary electrophoresis system. Accurate diffusion coefficient was obtained. The key conditions for the accurate measurement include: (1) systematic peak should be developed in a laminar flow; (2) radius diffusion is negligible compared with the longitudinal diffusion; (3) the capillary is well thermostated and (4) the solute should not be overloaded. In common case, the deviation of the measurement was kept within 4%. |
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| Keywords: | Minute protein Diffusion coefficient Capillary electrophoresis Taylor analysis |
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