Overview of chromatographic and electrophoretic methods for the determination of branched-chain amino acids |
| |
| Authors: | Xiongwei Yin Erwin Adams Ann Van Schepdael |
| |
| Affiliation: | Department of Pharmaceutical and Pharmacological Sciences, KU Leuven - University of Leuven, Pharmaceutical Analysis, Leuven, Belgium |
| |
| Abstract: | The significance of branched-chain amino acids in diseases was clearly shown over the years. This review aims to describe the available techniques for their analytical determination. The article provides examples of the use of various analytical methods. The methods are divided into two categories: derivatization and non-derivatization approaches. Separation is achieved through different chromatography or capillary electrophoresis techniques and can be combined with different detectors such as flame ionization, ultraviolet, fluorescence, and mass spectrometry. It compares the application of various derivatization reagents or detection as such for different detectors. |
| |
| Keywords: | analysis branched-chain amino acids derivatization review |
|
|
