Institution: | 1. Centre for Biomedical Engineering, University of Surrey, Guildford, UK;2. Centre for Biomedical Engineering, University of Surrey, Guildford, UK
DNAe, London, UK;3. Centre for Biomedical Engineering, University of Surrey, Guildford, UK
Department of Electrical Engineering and Electronics, University of Liverpool, Liverpool, UK |
Abstract: | Microfluidic devices for dielectrophoretic cell separation are typically designed and constructed using microfabrication methods in a clean room, requiring time and expense. In this paper, we describe a novel alternative approach to microfluidic device manufacture, using chips cut from conductor–insulator laminates using a cutter plotter. This allows the manufacture of microchannel devices with micron-scale electrodes along every wall. Fabrication uses a conventional desktop cutter plotter, and requires no chemicals, masks or clean-room access; functional fluidic devices can be designed and constructed within a couple of hours at negligible cost. As an example, we demonstrate the construction of a continuous dielectrophoretic cell separator capable of enriching yeast cells to 80% purity at 10 000 cells/s. |