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A DNA Origami-based Bactericide for Efficient Healing of Infected Wounds |
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| Authors: | Tiantian Wu Hong Wang Run Tian Shuang Guo Yuhui Liao Prof. Jianbing Liu Prof. Baoquan Ding |
| Affiliation: | 1. CAS Key Laboratory of Nanosystem and Hierarchical Fabrication, National Center for Nanoscience and Technology, 100190 Beijing, China Molecular Diagnosis and Treatment Center for Infectious Diseases, Dermatology Hospital, Southern Medical University, 510091 Guangzhou, China School of Pharmaceutical Sciences, Hainan Medical University, 570228 Haikou, China;2. CAS Key Laboratory of Nanosystem and Hierarchical Fabrication, National Center for Nanoscience and Technology, 100190 Beijing, China;3. CAS Key Laboratory of Nanosystem and Hierarchical Fabrication, National Center for Nanoscience and Technology, 100190 Beijing, China University of Chinese Academy of Sciences, 100049 Beijing, China;4. Molecular Diagnosis and Treatment Center for Infectious Diseases, Dermatology Hospital, Southern Medical University, 510091 Guangzhou, China |
| Abstract: | Bacteria infection is a significant obstacle in the clinical treatment of exposed wounds facing widespread pathogens. Herein, we report a DNA origami-based bactericide for efficient anti-infection therapy of infected wounds in vivo. In our design, abundant DNAzymes (G4/hemin) can be precisely organized on the DNA origami for controllable generation of reactive oxygen species (ROS) to break bacterial membranes. After the destruction of the membrane, broad-spectrum antibiotic levofloxacin (LEV, loaded in the DNA origami through interaction with DNA duplex) can be easily delivered into the bacteria for successful sterilization. With the incorporation of DNA aptamer targeting bacterial peptidoglycan, the DNA origami-based bactericide can achieve targeted and combined antibacterial therapy for efficiently promoting the healing of infected wounds. This tailored DNA origami-based nanoplatform provides a new strategy for the treatment of infectious diseases in vivo. |
| Keywords: | Bactericide DNA Origami Drug Delivery Infected Wound Healing Self-Assembly |