Kinetic determination of trace levels of selenium(IV) and total selenium by Nile Blue A/hydrogen peroxide method |
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Authors: | Gordana A. Milovanović Radivoj B. Petronijević Mira M. Čakar |
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Affiliation: | (1) Faculty of Chemistry, University of Belgrade, P. O. Box 158, 11001 Belgrade, Yugoslavia;(2) Institute for Technology of Nuclear and Other Mineral Raw Materials, Belgrade, Yugoslavia;(3) Faculty of Pharmacy, University of Belgrade, Belgrade, Yugoslavia |
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Abstract: | A kinetic method for the determination of selenium(IV) traces is proposed, based on its inhibitory action on the oxidation of Nile Blue A by hydrogen peroxide in phosphate buffer (pH 10.5). A linear dependence was established between the rate of the proposed indicator reaction and selenium concentration in the range 9.5 × 10–2-1.58 ng cm–3. The experimental conditions of maximal selenium effect were established. Selenium, determined by the tangent method, was determined at concentrations over the range 0.22–1.26 ng cm–3, with relative standard deviations up to 4.5%. The reaction rate was followed spectrophotometrically. The effect of foreign ions on the accuracy of this method was also investigated. The method was applied to the determination of selenium in pharmaceutical preparations and wheat flour. |
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Keywords: | selenium(IV) total selenium kinetic determination Nile Blue A oxidation hydrogen peroxide pharmaceutical preparations wheat flour |
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