A PDMS Device Coupled with Culture Dish for In Vitro Cell Migration Assay |
| |
| Authors: | Xiaoqing Lv Zhaoxin Geng Zhiyuan Fan Shicai Wang WeiHua Pei Hongda Chen |
| |
| Institution: | 1.State Key Laboratory of Integrated Optoelectronics, Institute of Semiconductors,Chinese Academy of Sciences,Beijing,China;2.College of Materials Science and Opto-Electronic Technology,University of Chinese Academy of Sciences,Beijing,China;3.School of Information Engineering,Minzu University of China,Beijing,China;4.State Key Laboratory of crystal materials,Shandong University,Jinan,China |
| |
| Abstract: | Cell migration and invasion are important factors during tumor progression and metastasis. Wound-healing assay and the Boyden chamber assay are efficient tools to investigate tumor development because both of them could be applied to measure cell migration rate. Therefore, a simple and integrated polydimethylsiloxane (PDMS) device was developed for cell migration assay, which could perform quantitative evaluation of cell migration behaviors, especially for the wound-healing assay. The integrated device was composed of three units, which included cell culture dish, PDMS chamber, and wound generation mold. The PDMS chamber was integrated with cell culture chamber and could perform six experiments under different conditions of stimuli simultaneously. To verify the function of this device, it was utilized to explore the tumor cell migration behaviors under different concentrations of fetal bovine serum (FBS) and transforming growth factor (TGF-β) at different time points. This device has the unique capability to create the “wound” area in parallel during cell migration assay and provides a simple and efficient platform for investigating cell migration assay in biomedical application. |
| |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ |
|
