Production of Bio-Ethanol by Integrating Microwave-Assisted Dilute Sulfuric Acid Pretreated Sugarcane Bagasse Slurry with Molasses |
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| Authors: | Na Yu Li Tan Zhao-Yong Sun Yue-Qin Tang Kenji Kida |
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| Institution: | 1.College of Architecture and Environment,Sichuan University,Chengdu,China;2.Civil Engineering Department,Sichuan Engineering Technical College,Deyang,China;3.Key Laboratory of Environmental and Applied Microbiology,Chengdu Institute of Biology, Chinese Academy of Sciences,Chengdu,China;4.Environmental Microbiology Key Laboratory of Sichuan Province,Chengdu,China |
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| Abstract: | Sugarcane bagasse (SCB) and molasses, known as carbohydrate-rich biomass derived from sugar production, can serve as feedstock for bio-ethanol production. To establish a simple process, the production of bio-ethanol through integration of whole pretreated slurry (WPS) of SCB with molasses was investigated. The results showed that microwave-assisted dilute sulfuric acid pretreatment reduced the formation of toxic compounds compared to a pretreatment process involving “conventional heating”. Pretreatment at 180 oC with 10% w v?1 solid loading and 0.5% w v?1 H2SO4 was sufficient to achieve efficient enzymatic saccharification of WPS. By conducting separate hydrolysis and fermentation (SHF), an ethanol yield of 90.12% was obtained from the mixture of WPS and molasses, but the ethanol concentration of 33.48 g L?1 was relatively low. By adopting fed-batch SHF, the ethanol concentration reached 41.49 g L?1. Assuming that the molasses were converted to ethanol at an efficiency of 87.21% (i.e., ethanol was obtained from fermentation of molasses alone), the ethanol yield from WPS when a mixture of WPS and molasses was fermented was 78.30%, which was higher than that of enzymatic saccharification of WPS (73.53%). These findings suggest that the production of bio-ethanol via integration of WPS with molasses is a superior method. |
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