Bioprocessing of Biodiesel Industry Effluent by Immobilized Bacteria to Produce Value-Added Products |
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| Authors: | Jyotsana Prakash Rahul Kumar Gupta Priyanka XX Vipin Chandra Kalia |
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| Affiliation: | 1.Microbial Biotechnology and Genomics, CSIR – Institute of Genomics and Integrative Biology (IGIB),Delhi University Campus,New Delhi,India;2.Academy of Scientific and Innovative Research (AcSIR),New Delhi,India |
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| Abstract: | Biodiesel industrial effluent rich in crude glycerol (CG) was processed to produce value-added product. Under continuous culture system, Bacillus amyloliquefaciens strain CD16 immobilized within its biofilm, produced 3.2 L H2/day/L feed, over a period of 60 days at a hydraulic retention time of 2 days. The effective H2 yield by B. amyloliquefaciens strain CD16 was 165 L/L CG. This H2 yield was 1.18-fold higher than that observed with non-biofilm forming Bacillus thuringiensis strain EGU45. Bioprocessing of the effluent released after this stage, by recycling it up to 25% did not have any adverse effect on H2 production by strain EGU45; however, a 25% reduction in yield was recorded with strain CD16. Biofilm forming H2 producers thus proved effective as self-immobilizing system leading to enhanced process efficiency. |
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