Efficient Malic Acid Production in <Emphasis Type="Italic">Escherichia coli</Emphasis> Using a Synthetic Scaffold Protein Complex |
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Authors: | Sivachandiran Somasundaram Gyeong Tae Eom Soon Ho Hong |
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Institution: | 1.Department of Chemical Engineering,University of Ulsan,Ulsan,Republic of Korea;2.Research Center for Bio-based Chemistry,Korea Research Institute of Chemical Technology (KRICT),Ulsan,Republic of Korea;3.Department of Green Chemistry and Environmental Biotechnology,Korea University of Science and Technology (UST),Daejeon,Republic of Korea |
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Abstract: | Recently, malic acid has gained attention due to its potential application in food, pharmaceutical, and medical industries. In this study, the synthetic scaffold complex strategy was employed between the two key enzymes pyruvate kinase (PykF) and malic enzyme (SfcA); SH3 ligand was attached to PykF, and the SH3 domain was attached to the C-terminus of ScfA. Synthetic scaffold systems can organize enzymes spatially and temporally to increase the local concentration of intermediates. In a flask culture, the recombinant strain harboring scaffold complex produced a maximum concentration of 5.72 g/L malic acid from 10 g/L glucose. The malic acid production was significantly increased 2.1-fold from the initial culture period. Finally, malic acid production was elevated to 30.2 g in a 5 L bioreactor from recombinant strain XL-1 blue. |
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