Effect of Extracellular Tyrosinase on the Expression Level of P450, Fpr,and Fdx and Ortho-hydroxylation of Daidzein in <Emphasis Type="Italic">Streptomyces avermitilis</Emphasis>

We have reported that the expression of CYP105D7 in Streptomyces avermitilis produces 112.5 mg L^?1 of 7,3′,4′-trihydroxyisoflavone (3'ODI) in 15 h of the reaction time, when 7,4′-dihydroxyisoflavone (daidzein) is used as a substrate. Although production is significant, rapid degradation of 3'ODI after 15 h was observed in a whole-cell biotransformation system, suggesting the further modification of 3'ODI by endogenous enzymes. In this present study, the effect of deletion of extracellular tyrosinase (melC2) in S. avermitilis for 3'ODI production as well as the expressions of CYP105D7, ferredoxin (Fdx), and ferredoxin reductase (Fpr) were investigated. The result revealed that daidzein hydroxylation activity in the ?melC2 mutant decreased by 40% compared with wild-type S. avermitilis. Further, melC2 deletion significantly affects the messenger RNA (mRNA) expression profile of CYP105D7 and its electron transfer counterparts. Real-time PCR analysis of 9 Fdx, 6 Fpr, and CYP105D7 revealed a significant decrease in mRNA expression level compared to wild-type S. avermitilis. The result clearly shows that the decrease in daidzein hydroxylation activity is due to the lower expression level of CYP105D7 and its electron transfer counterpart in the ?melC2 mutant. Furthermore, melC2 deletion prevents the degradation of 3'ODI.