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SSR-Based DNA Fingerprinting and Diversity Assessment Among Indian Germplasm of <Emphasis Type="Italic">Euryale ferox</Emphasis>: an Aquatic Underutilized and Neglected Food Crop |
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| Authors: | Nitish Kumar Divya Shikha Swati Kumari Binod Kumar Choudhary Lokendra Kumar Indu Shekhar Singh |
| Institution: | 1.Centre for Biological Sciences (Biotechnology), School of Earth, Biological and Environmental Sciences,Central University of South Bihar,Patna,India;2.Centre for Education (Life Science),Central University of South Bihar,Gaya,India;3.ICAR-National Institute of Biotic Stress Management,Raipur,India;4.ICAR-Indian Institute of Wheat & Barley Research,Karnal,India;5.ICAR RCER-Research Centre for Makhana,Darbhanga,India |
| Abstract: | Euryale ferox is native to Southeast Asia and China, and it is one of the important aquatic food crops propagated mostly in eastern part of India. The aim of the present study was to characterize and evaluate the genetic diversity of ex situ collections of E. ferox germplasm from different geographical states of India using microsatellite (simple sequence repeats (SSRs)) markers. Ten SSR markers were analyzed to assess DNA fingerprinting and genetic diversity of 16 cultivated germplasm of E. ferox. Total 37 polymorphic alleles were recorded with an average of 3.7 allele frequency per primer. The polymorphic information content value varied from 0.204 to 0.735 with mean of 0.448. A high range of heterozygosity (Ho 0.228; He 0.512) was detected in the present study. The neighbor-joining (N-J) tree and the principle coordinate analysis showed that the germplasm divided in to three main clusters. The results of the present investigation comply that SSR markers are effective for computing genetic assessment of genetic diversity and similarity with classifying cultivated varieties of E. ferox. Evaluation of genetic diversity among Indian E. ferox germplasm could provide useful information for genetic improvement. |
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