Determination of fluoroquinolone antibacterials as N-acyl derivatives |
| |
Authors: | J. A. Morley L. Elrod Jr. |
| |
Affiliation: | (1) Physical Analytical Chemistry Department, Abbott Laboratories, 60064-4000 North Chicago, Illinois, USA |
| |
Abstract: | Summary A simple and quantitative method for the preparation and high performance liquid chromatography (HPLC) of N-acylated fluoroquinolones has been developed. The acylation procedure was performed with four different acid anhydrides and found to be applicable to several fluoroquinolones. The acylated derivatives were chromatographed directly on a Nucleosil C18 column without the necessity of further sample manipulation. Detector response for the N-acetyl derivatives of norfloxacin, ciprofloxacin, sarafloxacin, and temafloxacin was linear to at least 50 g/mL. The retention behavior of the N-acyl derivatives can be predicted by the length of the carbon chain of the amide. |
| |
Keywords: | Column liquid chromatography Fluoroquinolones N-Acyl derivatives of fluoroquinolones Retention vs, structure |
本文献已被 SpringerLink 等数据库收录! |