新型固液双重致孔球形离子交换剂的制备及其性能研究

以甲基丙烯酸缩水甘油酯为单体 ,采用固液联合致孔方式 ,通过一步悬浮聚合制备了一种新型双孔高分子球形载体 .经化学修饰后 ,得到含二乙胺羟丙基的阴离子交换剂 (介质A) .优化了制备条件 .并与用相同方法制备的但仅含有机溶剂致孔剂的介质B进行了比较 .介质A和B均具有较高的静态吸附容量和机械强度 .由于介质A内含有流动相可以对流通过的大孔 ,因此其动态吸附容量远高于介质B ,并且在较高的流速下表现出较好的色谱流动性能

PREPARATION AND CHARACTERIZATION OF A NOVEL BIPOROUS SPHERICAL ION-EXCHANGER FOR PROTEIN ADSORPTION

A novel biporous spherical matrix of poly(glycidyl methacrylate-co-ethylene dimethacrylate) was prepared by suspension polymerization with superfine granules of calcium carbonate as solid porogenic agent and cyclohexanol plus dodecanol as solvent porogenic agents.After being functionalzed with diethylamine,the matrix(denoted as matrix A) was used as an anion exchanger for protein adsorption.The types and concentration of dispersing agents,the amount of calcium carbonate and solvent porogenic agents,as well as cross-linking agents for the preparation of the matrix had been optimized.It was found that in matrix A the macropores(>180 nm) took 37 9% of all the pore volume,while in the beads(matrix B) prepared with only solvent porogenic agents 86 8% of the pore volume was in the range 4～180 nm.Due to the presence of the macropores,convective flow occurred through matrix A in chromatography,leading to a high dynamic capacity up to 51 6 mg·g -1(wet resin) at a flow rate as high as 25 cm·min -1,nearly 4 times higher than that of matrix B.Moreover,the column of matrix A showed much lower backpressure than that of matrix B.These results indicated the potential application of the biporous matrix in high-speed chromatography for protein separation.