光合细菌产生5-氨基乙酰丙酸(ALA)的研究

从36个光合细菌菌株中筛选出的7株紫色非硫红假单胞菌有产ALA能力，其中菌株99-28ALA产量最高 用紫外线对菌株99-28进行诱变处理，筛选出产量比野生菌株高1倍多的菌株L-1，对影响菌株的生长和ALA产量的因子进行了探索，抑制剂加入时间和加入次数对ALA产量有显著影响，在最佳条件下（pH7.5，培养对数生长期加入ALA脱水酶抑制剂乙酰丙酸30mmol，ALA生物合成前体甘氨酸30mmol和琥珀酸30mmol，3000lx光照），菌株L-1的ALA产量可达22.15mg/L。

Research on photosynthetic bacteria strain to biological formation of 5-aminolevulinic acid

In the laboratory 7 strains of rhodopseudomonas sp were selected from 36 photosynthetic bacteria strains. Rhodopseudomonas sp strain 99-28 had the highest ALA production in these 7 strains. Rhodopseudomonas sp 99-28 strain was mutated using ultraviolet radiation and a mutant strain L-1 was obtained, in which ALA production was higher than wild strain 99-28 by about 100%. The elements which affect strain 99-28 and L-1 ALA formation were studied. Under the optimal condition of ALA formation (pH of 7.5, in the condition of supplementation of ALAD inhibitor LA and precursors glycine and succinat, and light 3 000 lx) ALA formation of mutant L-1 was as high as 22.15 mg/L.