Fast separation of hen egg white protein with a phosphorylcholine type zwitterionic ion chromatography stationary phase |
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| Authors: | Qian Qu Xiu Juan Yu Xi Wu Fei Shi Li Li Wang |
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| Institution: | Key Laboratory of Synthetic and Natural Functional Molecular Chemistry of Ministry of Education, Institute of Modern Separation Science, Northwest University, Shaanxi Key laboratory of Modern Separation Science, Xi’an 710069, China |
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| Abstract: | In this work, a kind of preparation method of zwitterionic ion chromatography (ZIC) stationary phase modified with phosphorylcholine (PC) was obtained by hydrolyzing after bonding phosphorylcholine dichloride to diol-silica to better explore the characteristics of the PC groups as ZIC stationary phase ligand in simultaneous separation of acidic proteins and basic proteins. The results showed that two kinds of acidic proteins and three kinds of basic proteins can be separated completely, meanwhile, hen egg white was separated and purified and three kinds of egg white components ovalbumin, G2 ovoglobulin and ovotransfemin proteins were separated completely by one single step on PC-ZIC column, the purity of all proteins reached above 95%. PC-ZIC stationary phase was successfully improved with better separation capacity and selectivity than previously reported in this paper. |
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| Keywords: | Phosphatidylchline Zwitterionic ion chromatography Protein Hen egg white |
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