EXO1基因单核苷酸多态性与云南汉族人群胃癌的相关性研究

胃癌是一种由基因和环境因素相互作用、在世界范围内最常见的恶性肿瘤之一.该研究用PCR-RFLP的方法,在270名健康对照个体和239名胃癌患者共509人的云南汉族人群中,对核酸外切酶1(exonuclease 1,EXO1)基因rs1635517、rs1776177、rs851797、rs1635498和rs1776148五个单核苷酸多态(single nucleotide polymorphisms,SNPs)位点进行多态检测.结果采用SPSS16.0、PHASE、HaploView4.2软件进行数据统计分析,用Logistic模型在95%置信区间内进行相关性风险度测算.结果显示,位于EXO1基因5UTR的位点rs1635517的CC基因型携带者和C等位基因携带者患胃癌的相对风险度显著升高(p＜0.05),提示该多态位点可能与胃癌发生相关.其余4个位点虽检测到多态,但它们的基因型和等位基因频率在病例和对照中无显著差异,提示这4个位点可能与云南汉族人群的胃癌发生不相关.

A study on relevance between EXO1 gene polymorphisms and gastric cancer in Han Population in Yunnan

Gastric cancer (GC) is one of the most common malignant cancers,which is influenced by both genetic and environmental factors.The relevance between five SNPs (rs1635517,rs1776177,rs851797,rs1635498,rs1776148) of EXO1 gene and gastric cancer (GC) was investigated in 509 individuals (270 healthy individuals and 239 patients with GC ) from Yunnan Han Population.Genotyping was performed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP).The statistical software package SPSS16.0,PHASE and HaploView4.2 was used.The results showed that both C allele and CC genotype of rs1635517 which located in the 5 UTR of EXO1 gene might be the risky factor of GC (adjusted p0.05), while the other 4 SNPs of EXO1 genes did not appear to influence the susceptibility to GC.