Aptamer/quantum dot-based simultaneous electrochemical detection of multiple small molecules |
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Authors: | Zhang Haixia Jiang Bingying Xiang Yun Zhang Yuyong Chai Yaqin Yuan Ruo |
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Institution: | aKey Laboratory on Luminescence and Real-Time Analysis, Ministry of Education, School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, PR China;bSchool of Chemistry and Chemical Engineering, Chongqing University of Technology, Chongqing 400040, PR China |
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Abstract: | A novel strategy for “signal on” and sensitive one-spot simultaneous detection of multiple small molecular analytes based on electrochemically encoded barcode quantum dot (QD) tags is described. The target analytes, adenosine triphosphate (ATP) and cocaine, respectively, are sandwiched between the corresponding set of surface-immobilized primary binding aptamers and the secondary binding aptamer/QD bioconjugates. The captured QDs yield distinct electrochemical signatures after acid dissolution, whose position and size reflect the identity and level, respectively, of the corresponding target analytes. Due to the inherent amplification feature of the QD labels and the “signal on” detection scheme, as well as the sensitive monitoring of the metal ions released upon acid dissolution of the QD labels, low detection limits of 30 nM and 50 nM were obtained for ATP and cocaine, respectively, in our assays. Our multi-analyte sensing system also shows high specificity to target analytes and promising applicability to complex sample matrix, which makes the proposed assay protocol an attractive route for screening of small molecules in clinical diagnosis. |
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Keywords: | Aptamers Multi-analyte detection Quantum dots Small molecules Square wave voltammetry |
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