Universal optical assays based on multi-component nanoprobes for genomic deoxyribonucleic acid and proteins |
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| Authors: | Li Jiang Wan Ying Wang Lihua Zhu Xinhua Su Yan Li Di Zhao Yun Huang Qing Song Shiping Fan Chunhai |
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| Institution: | aShanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, PR China;bSchool of Mechanical Engineering, Nanjing University of Science and Technology, Nanjing 210094, PR China;cSchool of Life Sciences, Sichuan University, Sichuan 610064, PR China |
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| Abstract: | In this report, we developed a universal assay method for both genomic DNA and proteins by using enzyme-based multi-component optical nanoprobes. The nanoprobes are gold nanoparticles assembled with bio-recognizing and signaling elements. We firstly demonstrated that the nanoprobes could detect unpurified asymmetric polymerase chain reaction (PCR) product from genomic DNA of Escherichia coli, with the sensitivity approximately 10 times higher than that of quantitative real-time PCR assay. The limit of detection (LOD) of our nanoprobe-based method is less than 10 pg template DNA (target DNA). Using DNA aptamers as recognition elements, we also showed that as few as 0.1 nM thrombin could be colorimetrically detected with high specificity. These results indicated that the enzyme-based multi-component nanoprobes have the capability to work with real biological samples, and have the potential in various biological and clinical applications. |
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| Keywords: | Multi-component Nanoprobe Gold nanoparticles Assymmetric polymerase chain reaction Aptamers |
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