Development of a combined technique using a rapid one-step immunochromatographic assay and indirect competitive ELISA for the rapid detection of baicalin |
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| Authors: | Paudel Madan Kumar Putalun Waraporn Sritularak Boonchoo Morinaga Osamu Shoyama Yukihiro Tanaka Hiroyuki Morimoto Satoshi |
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| Institution: | aDepartment of Pharmacognosy, Graduate School of Pharmaceutical Sciences, Kyushu University, Fukuoka 812-8582, Japan;bFaculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen, 40002, Thailand;cFaculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok 10330, Thailand;dFaculty of Pharmaceutical Sciences, Nagasaki International University, Huis Ten Bosch 2825-7, Sasebo, Nagasaki 859-3298, Japan |
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| Abstract: | A colloidal gold conjugated anti-baicalin monoclonal antibody (anti-BA MAb) was prepared and used in an immunochromatographic assay (ICA) for BA in Scutellariae Radix and Kampo medicines. This competitive ICA uses an anti-BA MAb which shows a high specificity for BA and baicalein. Its advantages include a short assay time (15 min), no dependence on any instrumental systems, and it can detect BA in plant materials and Kampo medicines. The limit of detection for the ICA was found to be around 0.6 μg mL−1of baicalin. Moreover, the usefulness of the combination of indirect competitive ELISA and the ICA using anti-BA MAb as a quality control method was confirmed for analysis of BA in Scutellariae Radix and Kampo medicines with a sufficient sensitivity (200 ng mL−1 to 2 μg mL−1), obtainable in an easy and timely manner. |
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| Keywords: | Baicalin Scutellaria Radix Immunochromatographic strip Anti-baicalin monoclonal antibody Enzyme-linked immunosorbent assay |
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