A novel lipase-based stationary phase in liquid chromatography |
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| Authors: | He Zeying Lv Chunguang Fan Xinxin Zhou Zhiqiang |
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| Institution: | aState Key Laboratory of Analytical Chemistry for Life Science, Department of Chemistry, Nanjing University, Nanjing 210093, PR China;bCollege of Chemistry and Engineering, Yangzhou University, 88 South University Avenue, Yangzhou 225002, PR China;cJiangsu Institute of Cancer Prevention and Cure, Nanjing 210009, PR China |
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| Abstract: | A streptavidin functionalized capillary immune microreactor was designed for highly efficient flow-through chemiluminescent (CL) immunoassay. The functionalized capillary could be used as both a support for highly efficient immobilization of antibody and a flow cell for flow-through immunoassay. The functionalized inner wall and the capture process were characterized using scanning electron microscopy. Compared to conventional packed tube or thin-layer cell immunoreactor, the proposed microreactor showed remarkable properties such as lower cost, simpler fabrication, better practicality and wider dynamic range for fast CL immunoassay with good reproducibility and stability. Using α-fetoprotein as model analyte, the highly efficient CL flow-through immunoassay system showed a linear range of 3 orders of magnitude from 0.5 to 200 ng mL−1 and a low detection limit of 0.1 ng mL−1. The capillary immune microreactor could make up the shortcoming of conventional CL immunoreactors and provided a promising alternative for highly efficient flow-injection immunoassay. |
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| Keywords: | Immune microreactor Capillary Chemiluminescence Immunoassay Streptavidin α-Fetoprotein |
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