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Ultra-sensitive DNA assay based on single-molecule detection coupled with fluorescent quantum dot-labeling and its application to determination of messenger RNA |
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| Authors: | Li Li Xincang Li Lu Li Jinxing Wang Wenrui Jin |
| Institution: | aSchool of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, China;bSchool of Life Sciences, Shandong University, Jinan 250100, China |
| Abstract: | |
| Keywords: | Abbreviations: BSA bovine serum albumin DEPC diethypyrocarbonate DNase deoxyribonuclease dNTP deoxy-ribonucleoside triphosphate DSC decidual stromal cell GOPS 3-glycidoxypropyltrimethoxysilane mRNA messenger RNA OPN osteopontin PBS physiological buffer saline pDNA probe DNA QD quantum dot QD-pDNA QD-labeled probe DNA R6G rhodamine 6G RNase ribonuclease rRNAsin RNase-inhibitor SA-QD Streptavidin-coated QD SDS sodium dodecyl sulfate SMD single-molecule detection tDNA target DNA TIRFM total internal reflection fluorescence microscopy |
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