Immunoassay of C-reactive protein by hot electron induced electrochemiluminescence using integrated electrodes with hydrophobic sample confinement |
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Authors: | Ylinen-Hinkka T Niskanen A J Franssila S Kulmala S |
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Affiliation: | aLaboratory of Analytical Chemistry, Aalto University School of Chemical Technology, P.O. Box 16100, FI-00076 Aalto, Finland;bDepartment of Materials Science and Engineering, Aalto University School of Chemical Technology, P.O. Box 16200, FI-00076 Aalto, Finland |
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Abstract: | C-reactive protein (CRP) was determined in the concentration range 0.01–10 mg L−1 using hot electron induced electrochemiluminescence (HECL) with devices combining both working and counter electrodes and sample confinement on a single chip. The sample area on the electrodes was defined by a hydrophobic ring, which enabled dispensing the reagents and the analyte directly on the electrode. Immunoassay of CRP by HECL using integrated electrodes is a good candidate for a high-sensitivity point-of-care CRP-test, because the concentration range is suitable, miniaturisation of the measurement system has been demonstrated and the assay method with integrated electrodes is easy to use. High-sensitivity CRP tests can be used to monitor the current state of cardiovascular disease and also to predict future cardiovascular problems in apparently healthy people. |
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Keywords: | Integrated microelectrode chip Hot electron induced electrochemiluminescence C-reactive protein Immunoassay |
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