| TTC-脱氢酶活性测定中标准曲线的影响因素研究 |
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| 引用本文： | 赵连梅,池勇志,张春青.TTC-脱氢酶活性测定中标准曲线的影响因素研究[J].实验室科学,2009(4):72-74. |
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| 作者姓名： | 赵连梅 池勇志 张春青 |
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| 作者单位： | 天津城市建设学院,环境与市政工程系,天津,300384 |
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| 摘 要： | 此文采用722S型可见分光光度计，以连二亚硫酸钠为还原剂，对传统TTC-脱氢酶活性测定法中影响标准曲线制作的因素进行了研究，确定了最佳测定条件为：最大吸收峰在475nm处；连二亚硫酸钠的最佳投量为5．0mg／5mL；11℃溶液的保存及整个实验过程应避光。最佳反应时间为30min。
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| 关 键 词： | TTC 脱氢酶活性 标准曲线 |
Study on influencing factors for standard curve of TTC-Dehydrogenase activity measurement |
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| ZHAO Lian-mei,CHI Yong-zhi,ZHANG Chun-qing.Study on influencing factors for standard curve of TTC-Dehydrogenase activity measurement[J].Laboratory Science,2009(4):72-74. |
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| Authors: | ZHAO Lian-mei CHI Yong-zhi ZHANG Chun-qing |
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| Institution: | ZHAO Lian - mei, CHI Yong - zhi, ZHANG Chun - qing (Department of Environmental and Municipal Engineering, Tianjin Institute of Urban Construction, Tianjin 300384, China) |
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| Abstract: | In this paper the factors which influence the standard curve of determination are investigated by using 722S type visible pectrophotometer and sodium hydrosulfite as reductant in the measure method conventional TIC - Dehydrogenase activity. The optimum conditions are as follows： the maximum absorption peak is at the point of 475 nm; the best dosage of sodium dithionite is 5. 0 mg/5mL ; preservation of TTC solution and the whole test process should be in darkness; the optimum reaction time is 30 rain. |
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| Keywords: | TTC |
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