State University of Ghent, Faculty of Pharmaceutical Sciences, Department of Pharmaceutical Chemistry and Drug Quality Control, Akademisch Ziekenhuis, De Pintelaan 135, B-9000 Ghent Belgium
Abstract:
The native fluorescence characteristics of domperidone in various solvents and at different pH values are reported. The fluorophore is the benzo-1,3-diazolin-2-one group which is also present in the analogous benperidol, droperidol and pimozide molecules from which domperidone is derived; the substituent may therefore be considered as a relatively strong fluorophore; its excitation and emission maxima are little influenced by pH variation. Domperidone can be determined in pharmaceutical preparations by its native fluorescence in ethanol (λex = 283 nm ;λem = 324 nm) and in 0.01 M HCl (λex = 284 nm;λem = 329 mn) with a detection limit of 0.01 μg ml-1.