高效液相色谱法测定氨基酸的研究进展

氨基酸是构成生物体的基础物质,氨基酸分析是生命科学研究中最重要的领域之一。高效液相色谱法因分析速度快、操作简便、检测灵敏、适用范围广等优点而广泛应用于食品工业、制药工业、生命科学研究等领域。该文综述了高效液相色谱分析氨基酸的方法,包括柱后衍生法、柱前衍生法、高效阴离子交换色谱-积分脉冲安培检测法和高效液相色谱-蒸发光散射检测法。并对上述方法进行了比较,为日常的氨基酸分析提供了参考。

Research Progress on Detection of Amino Acids by High Performance Liquid Chromatography

Amino acid is a fundamental material that organisms need,and its analysis is one of the most important technologies in life sciences.Owning the advantages of rapidness,easy operation,high detection sensitivity and wide application,the high performance liquid chromatography(HPLC) is widely used in many fields,such as food industry,pharmaceutical industry and life science research.In this article,the HPLC analytical methods of amino acids,including post column derivatization,pre column derivatization,high performance anion exchange chromatography with integrated pulsed amperometric detection and HPLC with evaporative light scattering detection,are reviewed.The characteristics of these methods are compared,which could provide a reference for the related research analysis.