How to approach preparative liquid chromatography |
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| Authors: | B. Coq G. Cretier C. Gonnet J. L. Rocca |
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| Affiliation: | (1) E. R. A. 474, Laboratoire de Chimie Analytique III, Université Claude Bernard-Lyon 1, 43 Boulevard du 11 Novembre 1918, F-69621 Villeurbanne, France |
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| Abstract: | Summary This work shows the influence of the injection volume on peak broadening owing to the increase of the sample concentration; use of the maximum injection volume is advocated. Once the chromatographic system is defined, a method for calculating the dimensions of the preparative column is developed. Applications of this method are shown. |
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| Keywords: | Preparative Liquid Chromatography Volume Overloading |
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