| 海带褐藻多糖硫酸酯的降解与岩藻寡糖的制备 |
| |
| 引用本文： | 耿丽华,金维华,王晶,张全斌.海带褐藻多糖硫酸酯的降解与岩藻寡糖的制备[J].高等学校化学学报,2017,38(12). |
| |
| 作者姓名： | 耿丽华 金维华 王晶 张全斌 |
| |
| 作者单位： | 1. 中国科学院海洋研究所实验海洋生物学重点实验室, 青岛 266071;中国科学院大学, 北京 100049;青岛海洋科学与技术国家实验室, 海洋生物学与生物技术功能实验室, 青岛 266071;2. 中国科学院海洋研究所实验海洋生物学重点实验室,青岛,266071;3. 中国科学院海洋研究所实验海洋生物学重点实验室, 青岛 266071;青岛海洋科学与技术国家实验室, 海洋生物学与生物技术功能实验室, 青岛 266071 |
| |
| 基金项目： | 国家自然科学基金，山东省重点研发计划项目(海洋医用食品)，山东省自然科学基金，青岛市应用基础研究计划-青年专项(批准号: 15-9-1-74-jch)资助. Supported by the National Natural Science Foundation of China，the Focus on Research and Development Plan in Shandong Province，China，the Shandong Provincial Natural Science Foundation of China，the Science and Technology Program of Applied Basic Research Projects of Qingdao Municipal |
| |
| 摘 要： | 采用稀酸水解的方法降解粗褐藻多糖硫酸酯(FPS)制备了硫酸化岩藻寡糖.通过考察降解及沉降条件研究了FPS的水解及沉降规律.通过理化性质分析发现,沉降所得上层清液为杂合硫酸化岩藻寡糖岩藻糖(Fuc)含量55%].进一步采用Bio-Gel P4低压凝胶渗透色谱分离纯化,得到4种以岩藻糖为主的低聚合度硫酸化寡糖.单糖组成分析和电喷雾质谱(ESI-MS)分析结果表明,各组分主要为系列低聚合度硫酸化岩藻寡糖或硫酸化岩藻糖,包括Fuc_(1~3)S_(1~3),Fuc_(1~2)S_(1~2),Fuc_(1~2)S_1及FucGlcAS.
|
| 关 键 词： | 褐藻多糖硫酸酯 岩藻寡糖 酸水解 |
Fucoidan Degradation and Preparation of Fuco-oligosaccharides from Saccharina japonica |
| |
| GENG Lihua,JIN Weihua,WANG Jing,ZHANG Quanbin.Fucoidan Degradation and Preparation of Fuco-oligosaccharides from Saccharina japonica[J].Chemical Research In Chinese Universities,2017,38(12). |
| |
| Authors: | GENG Lihua JIN Weihua WANG Jing ZHANG Quanbin |
| |
| Abstract: | The sulfated fuco-oligosaccharides were prepared via acid hydrolysis degradation of crude fucoidan extracted from Saccharina japonica. Through the optimization of degradation and precipitation conditions, the hydrolysis and sedimentation law of fucoidan were studied. Physicochemical analysis showed that the supernatant was heterozygous sulfated fuco-oligosaccharide(fucose>55%). Four fractions of fucose based low polyme-rization degree of oligosaccharides were purified by Bio-Gel P4 low-pressure permeation chromatogra-phy, and the fucose content of the fractions was up to 90% according to monosaccharide composition analysis and electrospray ionization mass spectrometry(ESI-MS) analysis. Thus, the fractions were series of low-poly-merized sulfated fuco-oligosaccharide / fucose, and the major fractions could be expressed with the formula of Fuc1~3 S1~3 , Fuc1~2 S1~2 , Fuc1~2 S, and FucGlcAS. |
| |
| Keywords: | Fucoidan Fuco-oligosaccharide Acid hydrolysis |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
