高性能量子点编码磁性微球的制备

基于改进的层层组装法,以氯仿/正丁醇混合溶液作为反应溶剂,将油溶性CdSSe/ZnS量子点装载到表面氨基修饰的磁性聚苯乙烯微球(MS)表面,通过调节量子点浓度,制备出高性能CdSSe/ZnS量子点编码磁性微球(CdSSe/ZnS-MBs).研究了氯仿和氯仿/正丁醇混合溶液对CdSSe/ZnS-MBs制备效果的影响.结果表明,氯仿/正丁醇混合溶液不仅能避免氯仿等量子点良溶剂对聚合物微球的形貌破坏,同时能促进CdSSe/ZnS量子点高效地装载到磁性微球表面.所制备的CdSSe/ZnS-MBs在水相具有较好的分散性,荧光强度变异系数(CV)小,形貌均一.该方法为简单、精确可控地制备高编码容量的量子点编码微球提供了新思路.

Preparation of High-Performance QD-encoded Magnetic Barcodes

The key element of the suspension array based multiplexed assays lies in the preparation of barcodes.Quantum dots(QDs) are ideal candidate fluorophores for optical encoding because of their outstanding fluorescent properties.By using chloroform/butanol mixed solution as CdSSe/ZnS QD reaction solvent,hydrophobic CdSSe/ZnS QDs were loaded onto the surface of magnetic polystyrene spheres via the modified layer-by-layer assembly proposed here.By the adjustment of QD concentration, high-performance CdSSe/ZnS QD encoded magnetic barcodes with different fluorescence intensity levels were successfully prepared.By comparing the CdSSe/ZnS QD encoded magnetic barcodes respectively prepared by using chloroform and chloroform/butanol mixed solution as reaction solvent, it was testified that the mixed solution helped to avoid the good QD solvent's damage to the morphology of polymer spheres and facilitated the assembly of CdSSe/ZnS QDs.The prepared CdSSe/ZnS QD encoded magnetic barcodes demonstrated good dispersity in water, narrow distribution of fluorescence intensity and uniform morphology.This method paves a new way for simple, accurate and controllable preparation of QD-encoded barcodes with high encoding capacity.