| 基于氧化石墨烯和DNA量子点组装体的DNA二元逻辑检测及循环可逆设计 |
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| 引用本文： | 沈晓琴,李智,王刚林,王莉,孙权洪,罗序成,马楠.基于氧化石墨烯和DNA量子点组装体的DNA二元逻辑检测及循环可逆设计[J].高等学校化学学报,2017,38(12). |
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| 作者姓名： | 沈晓琴 李智 王刚林 王莉 孙权洪 罗序成 马楠 |
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| 作者单位： | 苏州大学材料与化学化工学部,苏州,215123 |
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| 基金项目： | 国家自然科学基金，国家"八六三"计划项目(批准号: 2014AA020518) 和青年千人计划项目资助. Supported by the National Natural Science Foundation of China，the National High Technology Research and Development Program of China，the 1000-Young Talents Plan of China |
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| 摘 要： | 制备了水溶性的氧化石墨烯(GO)和以DNA为模板的Cd Te量子点(P1),通过GO与P1的π-π堆积作用组装构建了纳米生物传感器,将其用于双目标DNA分子的逻辑检测,实现了较高的选择性;通过改进DNA序列,实现了该传感器对双目标分子的可逆循环检测及重复利用.利用原子力显微镜(AFM)、透射电子显微镜、电泳和荧光光谱等方法对传感器的构建和检测过程进行了表征.该P1-GO纳米生物传感器在核酸检测等领域具有较大的应用前景.
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| 关 键 词： | 氧化石墨烯 DNA 荧光量子点探针 双分子识别 循环检测 |
Logic and Reversible Dual DNA Detection Based on the Assembly of Graphene Oxide and DNA-templated Quantum Dots |
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| SHEN Xiaoqin,LI Zhi,WANG Ganglin,WANG Li,SUN Quanhong,LUO Xucheng,MA Nan.Logic and Reversible Dual DNA Detection Based on the Assembly of Graphene Oxide and DNA-templated Quantum Dots[J].Chemical Research In Chinese Universities,2017,38(12). |
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| Authors: | SHEN Xiaoqin LI Zhi WANG Ganglin WANG Li SUN Quanhong LUO Xucheng MA Nan |
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| Abstract: | We prepared water-soluble monolayer graphene oxide( GO) and DNA-templated CdTe quantum dots(P1), which were further assembled via π-π stacking interaction to generate nano-biosensors for the logi-cal detection of dual DNA molecules with high selectivity. Moreover, reversible cyclic dual DNA detection is realized by changing DNA sequences, which enables regeneration of the biosensors. A variety of instruments were used to characterize biosensor preparation and detection process including atomic force microscopy (AFM), transmission electron microscopy(TEM), electrophoresis, fluorescence spectroscopy and so on. The prepared nano-biosensor holds great promise for nucleic acid detection. |
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| Keywords: | Graphene oxide DNA Fluorescent quantum dots probe Bimolecular recognition Cyclic detection |
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