| 基于1,8-萘酰亚胺的半胱氨酸荧光探针的合成及在生物成像方面的应用 |
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| 引用本文： | 杨敬敬,俞岳文,王炳祥,江玉亮. 基于1,8-萘酰亚胺的半胱氨酸荧光探针的合成及在生物成像方面的应用[J]. 高等学校化学学报, 2017, 38(7). DOI: 10.7503/cjcu20160960 |
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| 作者姓名： | 杨敬敬 俞岳文 王炳祥 江玉亮 |
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| 作者单位： | 江苏省生物功能材料重点实验室, 生物医药功能材料工程研究中心, 南京师范大学化学与材料科学学院, 南京 210046 |
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| 基金项目： | 国家自然科学基金，江苏高校优势学科建设工程项目资助.Supported by the National Natural Science Foundation of China，the Project Fund of the Priority Academic Program Development of Jiangsu Higher Education Institutions |
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| 摘 要： | 设计合成了一种用于检测半胱氨酸的新型荧光探针乙二醛(N-羟乙基-1,8-二甲酰亚胺-4-萘基)单腙(NAD),该荧光探针对半胱氨酸表现出较高的灵敏度和选择性.当半胱氨酸加入NAD溶液中,会形成分子内氢键,抑制C■N的异构化,导致荧光增强.此外,探针NAD可应用于细胞内半胱氨酸的检测,表明该类型探针在生物检测应用方面具有较大的潜力.
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| 关 键 词： | 荧光探针 半胱氨酸 细胞成像 1,8-萘酰亚胺衍生物 |
A Sensitive Fluorescent Probe for Cysteine Based on 1,8-Naphthalimide Derivative and Application in Bioimaging |
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| YANG Jingjing,YU Yuewen,WANG Bingxiang,JIANG Yuliang. A Sensitive Fluorescent Probe for Cysteine Based on 1,8-Naphthalimide Derivative and Application in Bioimaging[J]. Chemical Research In Chinese Universities, 2017, 38(7). DOI: 10.7503/cjcu20160960 |
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| Authors: | YANG Jingjing YU Yuewen WANG Bingxiang JIANG Yuliang |
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| Abstract: | A turn-on fluorescent probe glyoxal mono(N-hydroxyethyl-1,8-naphthalimide-4-hydrazone)(NAD) was designed and synthesized for the detection of cysteine(Cys).The fluorescent probe shows high selectivity and sensitivity toward Cys over other various amino acids.When Cys was added to the solution of the probe,the C=N isomerization process was inhibited by an intramolecular hydrogen bond,resulting in enhanced fluorescence.Furthermore,the probe NAD could effectively detect Cys in living cells. |
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| Keywords: | Fluorescent probe Cysteine Cells imaging 1 8-Naphthalimide derivative |
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