七元瓜环对2'-羟基查尔酮溶解性、稳定性及抗氧化活性的影响

利用~1H NMR、紫外吸收光谱和荧光光谱等方法考察了七元瓜环(Q7])对2'-羟基查尔酮(CET)的包结作用.结果表明,Q7]与CET形成了摩尔比为1∶1的包结配合物,紫外吸收光谱和荧光光谱测得的结合稳定常数分别为1.0248×10~6和1.253×10~6.相溶解度法研究结果表明,当Q7]的浓度为1×10~(-3)mol/L时,可使CET在水中的溶解度增加52倍.紫外吸收光谱随时间变化的研究结果表明,Q7]使CET的稳定性增加3.5倍.采用体外抗氧化活性测定(ABTS法)考察了Q7]对CET抗氧化活性的影响,发现CET@Q7]包结配合物以及游离CET均对ABTS自由基有较好的清除作用.IC50值分别为3.4×10~(-5)和2.4×10~(-5)mol/L,表明Q7]不仅能增加CET的溶解性和稳定性,同时对CET抗氧化活性的影响不大.

Influence of Interaction with Cucurbit [7] uril on Solubility,Stability and Antioxidant Activity of 2'-Hydroxychalcone

The host-guest interactions and properties of 2'-hydroxychalcone (CET) with cucurbit 7] uril (Q 7]) were investigated by means of 1H NMR,IR,UV-Vis absorption and fluorometry spectroscopy.The results revealed that 2'-hydroxychalcone could form an inclusion complex of 1 ∶ 1 with Q 7].The inclusion constants of C ET with Q 7] obtained by UV absorption and fluorescence spectroscopy were 1.0248× 106 and 1.253×106,respectively.The phase-solubility experiments revealed that the solubility of CET increased 52 times by interacting with Q7] c(Q 7])=1 × 10-3 mol/L].The time-scaled UV absorption spectra showed that Q 7] increases the stability of CET by 3.5 times.The antioxidant activity (ABTS method) of both the CET@Q7] inclusion complex and CET proved to be good scavenging effect to the ABTS radical,IC5o values were 3.4×10-5 and 2.4 × 10-5 mol/L,respectively.Thus,Q 7] could not only increase the solubility and stability of CET,but also have little effect on the antioxidant activity of CET.As a result,stability and water solubility were obviously improved.