万古霉素修饰的硅包银纳米三角片在耐万古霉素肠球菌拉曼成像和光动力学抑菌治疗中的应用

在银纳米三角片表面增强拉曼散射(SERS)活性基底表面包裹二氧化硅后再修饰卟啉-万古霉素单体化合物(Por-Van),制备了功能化的复合纳米颗粒(AgNPl@SiO_2-Por-Van).将该复合纳米颗粒用于耐万古霉素肠球菌(VRE)的拉曼成像和光动力学抑菌治疗.结果表明,该复合纳米颗粒能够与VRE耐药菌细胞壁间高效特异性结合,并且其体外光动力学抑菌活性均显著优于卟啉和卟啉-万古霉素单体.体外小鼠伤口感染实验进一步证实了其体内光动力学抑菌疗效.Por-Van修饰的复合纳米颗粒将拉曼成像和光动力学抑菌治疗有效整合,作为一种新型诊疗系统,可用于VRE耐药菌相关感染的监测及治疗.

Vancomycin Derivative Modified Silica-coated Silver Nanoplate for Surface-enhanced Raman Scattering Imaging and Antimicrobial Photodynamic Therapy of Vancomycin Resistant Bacterial Strains

Silver nanoplates, as SERS-active cores, were encapsulated by silica shell and modified with porphyrin-vancomycin(Por-Van) molecules to prepare AgNPl@SiO2-Por-Van composite.The Por-Van molecules conjugated on the AgNPl@SiO-2 core/shell nanostructure were applied for surface enhanced raman scattering(SERS) detection and antimicrobial photodynamic therapy(aPDT) of Vancomycin resistant enterococci(VRE) strains.Results for in vitro bacterial SERS imaging revealed that the core/shell nanostructure highly enhanced specific binding affinity towards VRE bacteria.Meanwhile, excellent bactericidal effect was also obtained through in vitro and in vivo photodynamic therapy studies.Therefore, the AgNPl@SiO2-Por-Van indicated high possibility for applications in theranostics towards VRE bacteria, integrating SERS imaging and aPDT.