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Radiolytic reactions of nitro blue tetrazolium under oxidative and reductive conditions: a pulse radiolysis study |
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| Authors: | A Kovacs L Wojnarovits M Baranyai A Moussa I Othman W L McLaughlin |
| Institution: | a Institute of Isotope and Surface Chemistry, Chemical Research Center, Hungarian Academy of Sciences, P.O. Box 77, Budapest H-1525, Hungary b Syrian Atomic Energy Commission, Damascus, Syria c National Institute of Standards and Technology, Gaithersburg, MD 20899, USA |
| Abstract: | The radiolytic reactions of the ditetrazolium salt nitro blue tetrazolium chloride (NBTCl2) were studied by pulse radiolysis technique in aqueous solution under reducing and oxidising conditions with the aim of potential dosimetry application. Under reducing conditions the fast formation of the tetrazolinyl radical is observed that is followed by the appearance of monoformazan (MF+), i.e. one of the tetrazolium rings is reduced to formazan. The formation of the water-insoluble diformazan, i.e. the result of the second reduction step was not observed in pulse radiolysis. Formazan formation was not found under oxidative conditions. |
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