Hydrodynamic chromatography separations in micro- and nanopillar arrays produced using deep-UV lithography (J. Sep. Science 35'15) |
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Authors: | Jeff Op de Beeck Wim De Malsche Piet De Moor Gert Desmet |
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Institution: | Department of Chemical Engineering, Vrije Universiteit Brussel, Brussel, Belgium. |
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Abstract: | DOI: 10.1002/jssc.201200076 Micro-pillar array columns with an inter-pillar spacing down to 500 nm have been designed and fabricated enabling hydrodynamic chromatography separations of fluorescently labeled polystyrene nano-particles. Injecting sub-nanoliter sized sample volumes and detecting the fluorescent signal on-chip, fluorescein, 20 and 40 nm diameter particles could be separated in less than 1.5 minutes. |
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