Mammalian cell growth studiedin situ by fourier transform infrared spectroscopy |
| |
Authors: | Timothy B Hutson Ming J W Chang Joseph T Keller David J Long |
| |
Institution: | (1) Battelle Columbus Division, The National Center for Biomedical Infrared Spectroscopy, 505 King Avenue, 43201 Columbus, OH, USA |
| |
Abstract: | Fourier transform infrared techniques were used to monitor mammalian cell growth plated on attenuated total reflection (ATR) crystals. Consistently, the growth kinetics plots generated from the obtained spectra demonstrated a sigmoidal curve representing a sequence of slow, rapid, then tapering cell growth which correlated well with non-spectral cell growth determinations. Significant advantages over traditional methods of measuring cell growth kinetics are demonstrated. The technique has promising potential to be used to study events in areas such as toxicology and cell biology. |
| |
Keywords: | FT-IR mammalian cell growth in situ ATR |
本文献已被 SpringerLink 等数据库收录! |