Field-amplified on-line sample stacking for determination of carnosine-related peptides by capillary electrophoresis |
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Authors: | Huang Ying Shi Yanfang Duan Jianping Chen Guonan |
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Institution: | The Key Laboratory of Analysis and Detection Technique for Food Safety, Ministry of Education, Department of Chemistry, Fuzhou University, Fuzhou, Fujian, PR China. |
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Abstract: | An on-line sample stacking method, namely field-amplified sample injection, has been developed for the separation and determination of carnosine, anserine, and homocarnosine by capillary electrophoresis. Using electrokinetic injection, about 130- to 160-fold improvement of sensitivity was achieved without loss of separation efficiency when compared to conventional sample injection. For conventional injection, the samples were dissolved in running buffer and then hydrodynamically injected for 10 s (3.45 kPa). Various parameters affecting separation and sample stacking were optimized. Under optimum conditions, linear responses were obtained over two orders of magnitude and the detection limits (defined as S/N = 3) of carnosine, anserine, and homocarnosine were 1.5 x 10(-8) to 1.6 x 10(-8) mol/L. |
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Keywords: | Capillary zone electrophoresis Carnosine‐related peptides Field‐amplified on‐line sample stacking |
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