| 尿毒症中分子毒物的分离鉴定 |
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| 引用本文： | 杨眉,张建达,王芃,刘晓航,袁直,刘宏富,杨洪涛,杨淑芬,赵菁丽. 尿毒症中分子毒物的分离鉴定[J]. 高等学校化学学报, 2005, 26(4): 677-679 |
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| 作者姓名： | 杨眉 张建达 王芃 刘晓航 袁直 刘宏富 杨洪涛 杨淑芬 赵菁丽 |
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| 作者单位： | 1. 吸附分离功能高分子材料国家重点实验室, 南开大学高分子化学研究所, 天津 300071;2. 天津市天和医院, 天津 300050;3. 天津市中医学院第一附属医院, 天津 300193 |
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| 基金项目： | 国家重点基础研究发展计划(973计划) |
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| 摘 要： | 自从1972年Babb等提出中分子假说以来，人们做了很多努力去分离鉴定尿毒症中分子毒性物质．Chu等通过凝胶色谱和离子交换色谱发现来自尿毒症患者血清和正常人尿液的A-3亚峰物质均含有6种成分，分子量分别为839．69，873．69，1007．94，1106．67，1680．28和2015．16．该组分在病人尿液中明显缺失，说明A-3亚峰物质可能是正常人通过尿液排出，肾衰病人难以通过尿液排出，导致在体内积累的物质；
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| 关 键 词： | 尿毒症 中分子 高效液相色谱 飞行时间质谱 |
| 文章编号： | 0251-0790(2005)04-0677-03 |
| 收稿时间： | 2004-05-14 |
Separation and Identification of the Uremic Middle Molecules |
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| YANG Mei,ZHANG Jian-Da,WANG Peng,LIU Xiao-Hang,YUAN Zhi,LIU Hong-Fu,YANG Hong-Tao,YANG Shu-Fen,ZHAO Jing-Li. Separation and Identification of the Uremic Middle Molecules[J]. Chemical Research In Chinese Universities, 2005, 26(4): 677-679 |
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| Authors: | YANG Mei ZHANG Jian-Da WANG Peng LIU Xiao-Hang YUAN Zhi LIU Hong-Fu YANG Hong-Tao YANG Shu-Fen ZHAO Jing-Li |
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| Affiliation: | 1. The State Key Laboratory of Functional Polymer Materials for Adsorption and Separation, Institute of Polymer Chemistry, Nankai University, Tianjin 300071, China;2. Tianhe Hospital, Tianjin 300050, China;3. First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China |
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| Abstract: | Sera and urine from patients with severe uremia and healthy persons were separated by means of gel permeation chromatography on Sephadex G-15 column with N(C2H5)3-H2CO3 buffer as the eluent. Two middle molecular peaks(A and B) were detected at 206 nm in normal urine, uremic serum and uremic urine, but these two peaks were hardly observed in the profile of normal sera. In contrast, the absorption at 206 nm of fractions A and B from uremic urine were smaller than that of fractions A and B from normal urine. Fractions A from normal urine, uremic serum and urine were collected and resolved into 3 subpeaks at 254 nm by high performance liquid chromatography. Two of these subpeaks, A-Ⅰ and A-Ⅱ, were detected in uremic serum, normal urine and uremic urine. The results of MALDI-TOF-MS revealed that the fraction A-Ⅰ from both uremic serum and normal urine contained a component with molecular weight 1214, which could hardly be seen in the fraction A-Ⅰ of uremic urine. |
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| Keywords: | Uremia Middle molecule High performance liquid chromatography Time-of-flight mass spectrometry |
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