PHOTOTRANSFORMATION OF AGGREGATED FORMS OF PROTOCHLOROPHYLLIDE IN ISOLATED ETIOPLAST INNER MEMBRANES |
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| Authors: | Bela Boddi Agneta Lindsten Margareta Ryberg Christer Sundqvist |
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| Affiliation: | Department of Plant Physiology, Eötvös Lóránd University, Budapest, Múzeum krt 4/A, 1088 Budapest, Hungary;Botanical Institute, Department of Plant Physiology, University of Göteborg, Carl Skottsbergs Gata 22, S-413 19 Göteborg, Sweden |
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| Abstract: | Abstract— Irradiation of an etioplast inner membrane fraction caused the transformation of two photoactive spectroscopically different protochlorophyllide forms into two chlorophyllide forms. A weak light flash, 6% of a saturating flash, preferentially caused the formation of a short wavelength chlorophyllide form absorbing at 672 nm and emitting at 676 nm. A saturating flash resulted in the formation of the 684 nm absorbing form of chlorophyllide with an emission maximum at 698 nm.
The circular dichroism (CD) signals of the newly formed chlorophyllide forms indicated that they are pigment aggregates of different sizes. These aggregates are probably connected to protochlorophyllide reductase and NADPH or NADP. In the absence of NADPH a decomposition of the pigment aggregates took place as revealed by a decrease in the CD-signal. A model is suggested which describes the structural changes of the pigment-protein aggregates after irradiation. |
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