Preparative isolation and purification of sinomenine from Sinomenium acutum by centrifugal partition chromatography |
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Authors: | Cheong Jong Hye Kim Chul Young Kim Jinwoong |
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Affiliation: | College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University, Seoul, Korea. |
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Abstract: | Preparative centrifugal partition chromatography (CPC) was successfully carried out for the separation of sinomenine from the methanolic extract of Sinomenium acutum stems and rhizomes. The optimum two-phase solvent system of CPC was composed of n-hexane/ethyl acetate/methanol/water at a volume ratio of 1:6:2:8 (v/v/v/v) with 0.1% triethylamine (TEA). Preparative CPC yielded 44.3 mg of sinomenine from 400 mg of MeOH extract with a purity of 96.9%. |
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Keywords: | Centrifugal partition chromatography Sinomenine Sinomenium acutum |
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