Evaluation of a high-performance thin-layer chromatographic technique for the determination of salbutamol serum levels in clinical trials. |
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| Authors: | A M Le Roux C A Wium J R Joubert P P Van Jaarsveld |
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| Affiliation: | Department of Pharmacology, Faculty of Medicine, University of Stellenbosch, Tygerberg, South Africa. |
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| Abstract: | Salbutamol concentrations were determined by high-performance thin-layer chromatography in the sera of two sets of ten volunteers at hourly intervals for 6 h after taking one 8-mg slow-release tablet. The influence of time lapse in processing of serum samples, i.e. centrifugation, extraction and chromatography, was studied. A statistical significant instability of salbutamol in the sera of patients was found which was not present in standard drug-free serum samples spiked with salbutamol and used for construction of standard curves. |
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