王老吉广东凉茶颗粒的超高效液相色谱指纹图谱研究

建立了适用于王老吉广东凉茶颗粒质量控制的超高效液相色谱指纹图谱分析方法。样品采用甲醇超声萃取30 min,萃取液用超高效液相色谱法进行指纹图谱分析。色谱柱采用Waters ACQUITY HSS T3 C18(3.0mm×150 mm,1.8μm),以0.5%甲酸-乙腈为流动相进行梯度洗脱,流速为0.8 mL/min,柱温35℃。28个共有峰在18 min内得到良好分离,通过对照品对其中4个峰进行了确证。对王老吉广东凉茶颗粒样品进行了相似度分析,23个批次样品的相似度均达到0.95以上;以28个共有峰的相对峰面积进行主成分分析,23批次样品均落在同一区域。相似度评价和主成分分析结果均表明,王老吉广东凉茶颗粒的产品质量稳定性好。该方法快速、高效、稳定,可用于王老吉广东凉茶颗粒的质量控制。

Fingerprint Analysis of Wanglaoji Guangdong Herbal Tea Granule by Ultra High Performance Liquid Chromatography

A fingerprint analysis method was developed for the quality control of Wanglaoji Guangdong herbal tea granule(GHTG)by ultra high performance liquid chromatography(UHPLC).The samples were extracted for 30 min by ultrasonic extraction using methanol as solvent.UHPLC separation was performed on a Waters ACQUITY HSS T3 C18(3.0 mm× 150 mm,1.8 μm) column by gradient elution with 0.5% formic acid-acetonitrile as mobile phase,at the temperature of 35 ℃ and a flow rate of 0.8 mL/min.A good separation of 28 common peaks was achieved within 18 min,and 4 of them were confirmed by reference substances.Similarity evaluation was performed by a professional software named Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine(Version 2004A),and the similarity among 23 batches of samples was no less than 0.95.The principal component analysis(PCA)also showed that the 23 batches of samples were located in one area.The results of similarity evaluation and PCA confirmed that the quality of Wanglaoji GHTG was stable,and the developed fingerprint analysis was fast,efficient and robust for the quality control of Wanglaoji GHTG.