高效液相色谱法快速检测氨基葡萄糖含量

应用高效液相色谱法，建立了硫酸氨基葡萄糖氯化钾胶囊中氨基葡萄糖含量的快速测定方法。采用Hedera NH2色谱柱（250 mm ×4.6 mm，5 μm），流动相为乙腈-磷酸缓冲液（ 65∶35）,流速为 1.0 mL/min，可变波长紫外检测器，检测波长为195 nm。 结果表明，在上述色谱条件下，氨基葡萄糖浓度在1.6~2.4 g/L范围内线性关系良好，检出限为7.81 mg/L,精密度（RSD,n=6）为0.53%；平均加标回收率为 100%,相对标准偏差为1.1%。该法准确、可靠，无需衍生反应，适用于硫酸氨基葡萄糖氯化钾胶囊中氨基葡萄糖的含量测定。

Rapid Determination of Glucosamine Content by High-performance Liquid Chromatography

A high-performance liquid chromatography(HPLC) method for the determination of glucosamine in glucosamine sulfate potassium chloride capsules was developed.The separation of analyte was performed on a Hedera NH2 column(250 mm ×4.6 mm,5 μm) with acetonitrile and phosphate buffer(65 ∶ 35) as mobile phase,at a flow rate of 1.0 mL/min.The variable wavelength detector(VWD) was set at 195 nm.In the above chromatographic conditions,the calibration curve was linear over glucosamine concentration in the range of 1.6-2.4 g/L.The limit of detection(LOD) of glucosamine was 7.81 mg/L.The RSD(n=6) for the accuracy was 0.53% and the average recovery was 100%with RSD of 1.1%.This method is simple,accurate and with no need of derivation,and is suitable for the determination of glucosamine.