| Binding equilibrium study of phosphotungstic acid and HSA or BSA with UV spectrum, fluorescence spectrum and equilibrium dialysis |
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| 作者姓名： | 黄瑾 袁余洲 梁宏 |
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| 作者单位： | HUANG Jin，YUAN Yuzhou & LIANG HongInstitute of bioinorganic Chemistry，Department of Chemistry，Guangxi Normal University，Guilin 541004，China |
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| 基金项目： | This work was supported by the National Natural Science Foundation of China (Grant No.
29961001)，the Cross-Century Talents Foundation of Guangxi Zhuang Autonomous Region and Science Foundation of Guangxi University. |
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| 摘 要： | The binding equilibrium between phosphotungstic acid (H7P(W2O7)6] · XH2O;PTA) and human serum albumin (HSA) or bovine serum albumin (BSA) has been studied by UV-Vis, fluorescence spectroscopies and equilibrium dialysis. It has been observed that UV absorption enhanced and the fluorescence quenched as the PTA binding to HSA or BSA at physiological pH 7.43(?.02). The Scatchard analysis indicated that there exists a strong binding site of PTA in both HSA and BSA, and the successive stability constants of these two systems are obtained by nonlinear least-squares methods fitting Bjerrum formula.
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| 收稿时间： | 11 November 2008 |
Binding equilibrium study of phosphotungstic acid and HSA or BSA with UV spectrum, fluorescence spectrum and equilibrium dialysis |
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| Jin Huang, Yuzhou Yuan and Hong Liang.Binding equilibrium study of phosphotungstic acid and HSA or BSA with UV spectrum, fluorescence spectrum and equilibrium dialysis[J].Science in China(Chemistry),2002,45(2):200-207. |
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| Authors: | Jin Huang Yuzhou Yuan and Hong Liang |
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| Institution: | Institute of bioinorganic Chemistry, Department of Chemistry, Guangxi Normal University, Guilin 541004, China |
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| Abstract: | The binding equilibrium between phosphotungstic acid (H7P(W2O7)6]@XH2O;PTA) and human serum albumin (HSA) or bovine serum albumin (BSA) has been studied by UV-Vis, fluorescence spectroscopies and equilibrium dialysis. It has been observed that UV absorption enhanced and the fluorescence quenched as the PTA binding to HSA or BSA at physiological pH 7.43( ± 0.02). The Scatchard analysis indicated that there exists a strong binding site of PTA in both HSA and BSA, and the successive stability constants of these two systems are obtained by nonlinear least-squares methods fitting Bjerrum formula. |
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| Keywords: | serum albumin phosphotungstic acid UV spectrum fluorescence spectrum equilibrium dialysis |
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