| Isolation and identification of nondestructive desulfurization bacterium |
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| 引用本文： | 缑仲轩,刘会洲,罗明芳,李珊,邢建民,陈家镛. Isolation and identification of nondestructive desulfurization bacterium[J]. 中国科学B辑(英文版), 2002, 45(5): 521-531. DOI: 10.1360/02yb9069 |
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| 作者姓名： | 缑仲轩 刘会洲 罗明芳 李珊 邢建民 陈家镛 |
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| 作者单位： | GOU Zhongxuan，LIU Huizhou，LUO MingfangLI Shan，XING Jianmin & CHEN JiayongYoung Scientist Laboratory of Separation Science and Engineering，State Key Laboratory of Biochemical Engineering，Institute of Process Engineering，Ch |
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| 基金项目： | This work was supported by the State Major Basic Research Development Program of China (Grant No. G2000048004)，SINO Petrochemical Corporation Ltd. (SINOPEC) (Grant No. X599009). |
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| 摘 要： | A nondestructive desulfurization microorganism has been isolated. The metabolism product analyses show that the strain can be a kind of biocatalyst to oxidize dibenzothiophene (DBT) into 2-hydroxydiphenyl (HBP), therefore the sulfur in DBT is removed selectively. The 16SrRNA information, cell wall analysis, physical, biochemical properties and morphological properties suggest that the isolated strain is Rhodococcus erythropolis. The strain can grow in the basal salts medium (BSM) that DBT concentration is no more than 10 mmol/L, and the optimal DBT concentration for growth is 1 mmol/L, however, the optimal DBT concentration for desulfurization is 0.5 mmol/L. The further research shows that the strain can also desulfur some other or-ganosulfur-containing compounds such as thianaphthene, phenyl sulfide and 4,6-dimethyldiben-zothiophene (4,6-DMDBT).
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| 收稿时间： | 2005-03-04 |
Isolation and identification of nondestructive desulfurization bacterium |
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| Zhongxuan Gou, Huizhou Liu, Mingfang Luo, Shan Li, Jianmin Xing and Jiayong Chen. Isolation and identification of nondestructive desulfurization bacterium[J]. Science in China(Chemistry), 2002, 45(5): 521-531. DOI: 10.1360/02yb9069 |
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| Authors: | Zhongxuan Gou Huizhou Liu Mingfang Luo Shan Li Jianmin Xing Jiayong Chen |
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| Affiliation: | Young Scientist Laboratory of Separation Science and Engineering, State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100080, China |
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| Abstract: | A nondestructive desulfurization microorganism has been isolated. The metabolism product analyses show that the strain can be a kind of biocatalyst to oxidize dibenzothiophene (DBT) into 2-hydroxydiphenyl (HBP), therefore the sulfur in DBT is removed selectively. The 16SrRNA information, cell wall analysis, physical, biochemical properties and morphological properties suggest that the isolated strain is Rhodococcus erythropolis. The strain can grow in the basal salts medium (BSM) that DBT concentration is no more than 10 mmol/L, and the optimal DBT concentration for growth is 1 mmol/L, however, the optimal DBT concentration for desulfurization is 0.5 mmol/L. The further research shows that the strain can also desulfur some other or-ganosulfur-containing compounds such as thianaphthene, phenyl sulfide and 4,6-dimethyldiben-zothiophene (4,6-DMDBT). |
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| Keywords: | dibenzothiophene isolation identification biodesulfurization Rhodococcus erythropolis. |
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