Separation of serum proteins with uncoupled microsphere particles in a stirred cell |
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Authors: | Jeong-Yeol Yoon Jung Hun Lee Jung-Hyun Kim Woo-Sik Kim |
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Institution: | Department of Chemical Engineering, College of Engineering, and Bioproducts Research Center, Yonsei University, 134 Shinchon-dong, Sudaemoon-ku, Seoul 120–749, South Korea |
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Abstract: | Submicron microspheres were used directly without ligand coupling for the batch and continuous separations of proteins. In the batch experiments for separating BSA (bovine serum albumin) from BHb (bovine hemoglobin), introducing both hydrophobic effects for BSA and electrostatic repulsion for BHb (and vice versa) was required for high selectivity, and microspheres with low number density of surface groups were advantageous. For the continuous experiments, the utilization of a stirred cell was successful, where the microspheres were in the form of latex with good dispersion of particles. The flow rate without a pump was 0.5–1.3 ml min?1, and the ratio of BSA and BHb was varied. In the experiments for eliminating BHb from BSA, elution curves of BHb corresponded to the single component breakthrough curves, while those for BSA did not. The latter is believed to be due to the interference by BHb in the adsorption of BSA. |
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Keywords: | Protein separation Microspheres Stirred cell Bovine serum albumin Bovine hemoglobin |
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