二维核磁共振对羊栖菜褐藻糖胶DJL的结构研究

羊栖菜是我国的特色海藻，褐藻糖胶是其主要生物活性成分. 葡萄糖醛酸甘露聚糖（DJL）是羊栖菜褐藻糖胶经脱硫和部分酸水解后的产物，本文采用2D NMR和甲基化分析等方法，对DJL的结构进行了研究. 结果显示，DJL主要由甘露糖（46.4%）和葡萄糖醛酸（50.5%）组成， 另外还含有少量的半乳糖，相对分子质量为11 KDa，其可能具有的一种结构为：β-D-GlcA-1→ 2)-α-D-Man(1→4)-β-D-GlcA(1→]_n1→4)-β-D-Gal(1→4)-β-D-GlcA(1→2)-α-D-Man(1→]_n2→4)-β-D-GlcA(1→2)-α-D-Man.

Structure Elucidation of Fucoidan DJL Originated from Hizikia fusiforme by 2D NMR

Fucoidan is the primary bio-active component in Hizikia fusiforme, an algae widely distributed in China. DJL is a product of Hizikia fusiforme-originated fucoidan after desulfation and partial hydrolysis. In this study, the structure of DJL was elucidated using 2D NMR spectroscopy and methylation analysis. The result shows that DJL has a molecular weight of 11 KDa and is composed of mannose (Man) and glucouronic acid (GlcA) with a composition ratio of about 1∶1. Its possible structure was also determined to be β-D-GlcA(1→ 2)-α-D-Man(1→4)-β-D-GlcA(1→]_n1→4)-β-D-Gal(1→4)-β-D-GlcA(1→2)-α-D-Man(1→]_n2→4)-β-D-GlcA(1→2)-α-D-Man.